Facilities Available for

US, CT, MRI, EchoMRI (mouse fat-water measurement), X-ray irradiator.

A state-of-the-art light microscopy facility specializing in live and fixed cell imaging. Specializes in Widefield, Spinning-Disk Confocal, Laser Scanning Confocal, Deconvolution and Super-Resolution fluorescence microscopy. Also advanced software for image analysis and presentation (Imaris, HuygensPro), as well as microtechnique (both paraffin/plastic and cryotomy) equipment and training.

The Cal-Cryo facility, housed in the basement of Stanley Hall, is led by Professors Eva Nogales and James Hurley and began operations in February 2018. Our facility is used to collect image data of biological or soft material samples before it is processed using single particle or tomographic reconstruction methods Our staff offer consultation and training in specimen preparation, microscope operation, image acquisition, and data analysis. The facility’s instrumentation features automated data collection. Both single-particle micrographs and tomography tilt series can be collected.

Cal-Cryo@QB3-Berkeley instruments include: 

• Krios G2 300kV CryoTEM
  GATAN K3 Direct Electron Detector
  Volta Phase Plate, BIO Quantum Energy Filter
•  Krios G3i 300kV CryoTEM
  GATAN K3 Direct Electron Detector
  BIO Quantum Energy Filter
• Talos Arctica 200kV CryoTEM
  Gatan K3 Direct Electron Detector
• FEI Mark IV Vitrobot

Request services or access through Second Lab

The Cell Culture Facility, located in 336 Barker Hall, supports research by providing cell cultures (mammalian and insect), media, supplies and expertise on a recharge basis for more than 70 different laboratories on and off the main campus. The Facility provides cell cultures for researchers to take to their labs for experiments, provides media and materials for checkout, and also allows use of laminar flow hoods, incubators and microscope for researchers who are using cells provided by the Facility, to use on site. We also work with off-site companies.

Accepts organic, soil, water, and air samples for analysis of stable isotopes of carbon, nitrogen, sulphur, oxygen and hydrogen at natural abundance.

The College of Chemistry Glass Shop produces custom glassware for research. Services include design, consulting on glassware systems, and custom glassware fabrication, with experience in producing glass apparatus for physics, chemistry, materials engineering, and other research focuses.

Equipment includes glassblowing lathes, torches, kiln, diamond saw and other glass coldworking equipment.

The Microanalytical Facility provides Elemental Analysis. Elemental analysis is a process where a sample of the some material (e.g. soil, water ,polymers, minerals, chemical compounds) is analyze for their chemical composition.  There are a several analytical instruments in the facility:

1. Perkin Elmer CHNS 2400 series II analyzer . The detection limit for C,H,N and S is 0.2%.
2. Thermo Fisher Flash Smart Elemental analyzer( C,H,N,S analysis) The detection limit 0.01%.
3. Perkin Elmer ICP Optima 7000 DV Spectrometer ( for metals analysis) The detection limit 0.01ppm. Open access instrument.

Provides all services related to the X-ray diffraction of single crystals and powders of small molecule compounds. Typically, X-ray diffraction is used to determine the solid-state molecular structure at the atomic level of crystalline samples, including absolute stereochemistry of chiral compounds. The facility currently houses state-of-the-art single crystal and powder X-ray diffractometers.

A state-of-the-art light microscopy facility specializing in imaging live cell and live small model organisms (zebrafish, drosophila, c. elegans, etc.), laser-scanning and spinning disk confocal microscopy, multi-photon microscopy, fluorescence lifetime imaging (FLIM), super resolution (AiryScan and Fast Airyscan), light-sheet microscopy (SPIM), holographic patterned illumination for optogenetic studies, and slide scanning with histological stains and fluorescence.

The CRL Molecular Imaging Center operates in 3 different buildings on campus, with the primary locations in Life Sciences Addition (251 LSA), a North-side core in 320 Barker Hall, and a small outpost in the Li Ka Shing Center for Biomedical and Health Sciences (230 Li Ka Shing Center). 

Serves all UC affiliated laboratories, academic institutions, government agencies and commercial businesses – providing Sanger sequencing, genotyping, high-throughput nucleic acid purification/quantification, PCR reaction cleanup, PCR products size selection, human cell line authentication, stem cell validation, and free (and paid) consultation services. Open access (for a fee) instruments include: Bio-Rad CFX96 Touch Real Time PCR System, Bio-Rad QX200 Droplet Digital PCR System, and an Agilent Technologies TapeStation 4200. 

The Earth and Planetary Sciences XRD, SEM, and Sample Preparation Labs provide users the opportunity to perform a wide range of analyses on samples including X-ray powder diffraction, SEM imaging, and EBSD textural analysis, EDS chemical analysis as well as all sample preparation required for analysis. 

Equipment includes: 

• Zeiss EVO MA-10 SEM with BSE and SE imaging capabilities, EDAX detector, EBSD detector;
• Panalytical X-pert Pro diffractometer with X-celerator detector;
• carbon evaporator (for coating samples);
• various rock cutting, grinding and polishing equipment;
• petrographic microscopes and binocular microscopes with photo imaging capabilities.

Applications include:

• SEM: Secondary electron imaging, backscattered electron imaging, EDS chemical analysis and mapping, Electron Backscatter Diffraction texture analysis;
• XRD: powder diffraction phase identification, thin film analysis.

Provides EM services to the Berkeley campus and the academic community at large. The EML houses both TEMs and SEMs and provides three types of service: (1) education and training, (2) equipment for trained users, and (3) full service microscopy for pilot studies. The EML specializes in cryopreservation via HPF/FS. All services are available to anyone with an interest in electron microscopy: graduate and undergraduate students, post-doctoral researchers, faculty, staff, and non-UC users.

The Flow Cytometry provides UC Berkeley and local researchers with access to cutting-edge High-Dimensional Fluorescence Activated Cell Sorting (Hi-D FACS) and analysis. The facility also provides flow cytometry instruments and expertise.

The College of Natural Resources' Inductively Coupled Plasma (ICP) Spectroscopy Facility has a Perkin Elmer 5300 DV optical emission ICP with auto sampler.  The ICP can measure up to at least 21 different elements simultaneously. Users should estimate between 6 and 12 samples per hour plus a half hour warm up of the instrument.  Applications include plant elemental analysis, soil extracts, silicon chip analysis, nanoparticles, rock fusions to name just a few. 

The Marvell NanoLab, located in the CITRIS headquarters building, Sutardja Dai Hall, includes more than 15,000 sq feet of Class100 and Class1000 cleanroom. The Marvell NanoLab is a shared research center providing more than 100 Principal Investigators and over 500 academic and industrial researchers a complete set of micro- and nano-fabrication tools. Please contact us if you are an academic or industrial researcher interested in the capabilities of the Marvell NanoLab.

Provides bacterial, yeast, C. elegans (worms), Drosophila, or any specialty media you have a protocol for on a recharge basis to over 20 labs on campus and at LBNL.

The MGCF is a research resource in computational chemistry and scientific visualization for over 50 research groups in the College of Chemistry and the wider campus community. We provide high-performance computing resources for research and educational uses in chemistry and related disciplines.

Greenhouse, growth chamber, lath house, and field space for plant and environmental science research. Resources include mist propagation beds and rooms, work space, supplemental lighting, and biosafety, temperature, and lighting controls. Experienced staff provides horticultural consultation, supplies, plant monitoring and watering, and pest control. The facility is available to UC Berkeley and LBL affiliates and, on a limited basis, to outside users.

The PEER Lab is located at the Richmond Field Station and has a long history of successfully providing the engineering community testing facilities, the staffing expertise to execute a given project, and the academic background to provide appropriate data analysis, design input and overall project management. The PEER-UC Berkeley Lab is available to write both academic style reports, along with AC156 and IEEE-693 reports submitted to regulatory agencies. 

A welding shop, machine shop and electronics shop, along with dedicated control rooms, conference rooms and a suite of offices are also located at the PEER-UC Berkeley Lab facility.

The PEER Lab is available to both the research community and to private industry that may require large capacity testing services. Published recharge rates are utilized in the development of project budgets. Priority in scheduling a given test always favors the research community, and time is made available to commercial clients on a time-available basis.

Provides routine access for liquid- and solid-state NMR experiments as well as diffusion and micro-imaging applications. PMRC Core is primarily used for determining critical aspects of molecular structure, for studying reaction mechanisms qualitatively and quantitatively, and materials characterization. Instruments are available at field strengths 300 MHz to 700 MHz with probes to study a broad-band of nuclei (1H-19F and 31P-103Rh).  Further information can be found at: pmrc.berkeley.edu

The BNC is a fabrication and experimentation facility specializing in BioMEMS, microfluidic devices, electron/ion beam microscopy and nanofabrication. We are a core facility of the California Institute for Quantitative Biosciences and are open to university, national lab, and industry users. Equipment and instrumentation encompasses photolithography, soft lithography, deposition, etching, metrology (including Keyence 3D laser scanning microscope), biological experimentation, an FEI Quanta dual-beam gallium FIB/SEM (EDX, EBSD, STEM detector, OmniProbe, gas injector system, pico-indenter, environmental mode), and a Zeiss ORION NanoFab Helium Ion Microscope (gas injector system for insulator/metal deposition and gas-assisted etching, charge-neutralization system, gallium FIB, NPVE pattern generator).  Instrumentation also includes PHI XPS (X-ray Photoemission Spectroscopy) with ion beam for materials analysis, both surface and bulk properties, Nanosurf COREAFM  atomic force microscopy, and Parylene Chemical Vapor Deposition system.  A full list of equipment and services can be found on the BNC website.  The BNC also houses instructional labs and is adjacent to QB3 incubator space.

Request services or access through Second Lab

The Cell and Tissue Analysis Facility (CTAF) provides the cell culture equipment and high-end instrumentation needed to grow and assess mammalian cells, tissues, proteins, and more from flow cytometry to multiplex ELISA to automated epifluorescence, confocal, and multiphoton imaging.

Instruments include:

• Cell Culturing Facility for Mammalian Cell Culture with Biospherix Hypoxia Chambers
• Automated Epifluorescence/Live Imaging Microscopy and Analysis Workstation; ImageXpress Micro, Molecular Devices
• Lionheart FX/Live Imaging, Agilent/Biotek
• Multiphoton Photon Microscopy; Bruker/Prairie Technologies
• Confocal Microscopy, Bruker/Prairie Technologies
• SpectraMax M5 MultiMode Plate Reader, Molecular Devices
• Bio-Plex MAGPIX Multiplex Reader, BioRad
• Flow Cytometry; Attune NxT Acoustic Focusing Cytometer blue/red/violet/yellow, ThermoFisher
• CFX Connect qPCR, Thermal Cycler, and GenePulser Xcell Electroporator, BioRad
• Histology Suite with Cryostar NX50 cryostat, HM 430 Freezing Stage Sliding Microtome, and Paraffin Embedding (FFPE) and Sectioning, ThermoFisher 

This facility was formerly titled the QB3 Shared Stem Cell Facility.

Promotes the understanding of the structures and dynamics of natural products, potential therapeutics, proteins, and nucleic acids at the atomic level. Instruments include a 900 MHz spectrometer equipped with a cryoprobe, along with several other NMR systems.

Provides routine acquisition of mass spectra and accurate mass measurements of biological, organic, and inorganic compounds, as well as mass measurements of intact proteins, lipids, oligosaccharides, and non-covalent protein-protein and protein-ligand complexes and supramolecular coordination clusters. The facility also provides advanced proteomics analysis and operates an open access laboratory. 

The facility provides acquisition of mass spectra and accurate mass measurements of biological, organic and inorganic compounds, as well as mass measurements of intact proteins, lipids, oligosaccharides, and non-covalent protein-protein and protein-ligand complexes and supramolecular coordination clusters. The facility also provides advanced proteomics analysis. This involves protein identification using mass spectral data as well as identification of posttranslational modifications. In addition, the facility provides ion mobility spectrometry. The facility also operates an open access MALDI-TOF instrument available to trained users.

QB3 Genomics is a partnership between the Vincent J. Coates Genomics Sequencing Lab ( GSL – B206 Stanley Hall), the Functional Genomics Laboratory (FGL – 255 Weill Hall, formerly LSA), and the Computational Genomics Resources Facility (CGRL – 238 Koshland Hall). QB3 Genomics’ mission is to make genomics research tractable and affordable to UC Berkeley and the greater genomics community.

The VCGSL provides QC and sequencing services. We operate Illumina MiSeq, HiSeq, and NovaSeq platforms, as well as a PacBio Sequel II system and Oxford Nanopore Minion system. The GSL also provide PacBio and ONT library preps and high molecular weight extractions.

The FGL provides molecular biology services for next-generation sequencing projects, including library preparation, QC, purification, enrichment, and single cell (10X) services.

The CGRL provides shared computational infrastructure and technical support in collaboration with Berkeley Research Computing. The CGRL also provides consulting for bioinformatics and genomic analyses.

For more detailed information on services, submissions, and rates please visit the QB3 Genomics.

The High-Throughput Screening Facility (HTSF), located in the Li Ka Shing Center for Biomedical and Health Sciences, provides access to cell culturing space, automated liquid handling, automated plate reader and high-throughput, high-content microscopy instrumentation for screening experiments, along with tools for analysis and data visualization. Emphasis is on whole genome and sub-library siRNA screening from various sources but other high-throughput fluidics projects are welcome.

Instruments include:

• Combidrop/Multidrop, ThermoFisher
• V11 Bravo Automated Liquid Handler, Agilent with Inline Plate Washer and Sealer 
• BioMek NX with Span-8, Beckman-Coulter
• Muitiflo FX MultiMode Dispenser, Agilent/Biotek
• EVOS FL Color Imaging System, ThermoFisher
• Envision MultiMode Plate Reader, Perkin-Elmer
• Automated Confocal/Wide-field/Transmitted Light Microscope Opera Phenix, Perkin Elmer with Incubator and Robotic plate handler for Live Imaging
• UCB only: Lentiviral Packaging, Addgene CRISPR library amplification and verification Service
• UC only: Synthego CRISPR Knockout (KO) reagents

Provides comprehensive proteomics services using mass spectrometry, determining the protein contents of samples as simple as gel bands or as complicated as whole cell extracts. The facility provides identification of post-translational modifications and relative quantitation as well as consultation regarding sample preparation and experimental design.

The facility provides proteomics services principally through mass spectrometry.  Currently we have two Thermo LTQ XL linear ion trap mass spectrometers and a Thermo Fusion Lumos mass spectrometer.

Request services or access through Second Lab