Cal-Cryo@QB3Berkeley RIC

The Cal-Cryo facility, housed in the basement of Stanley Hall, is led by Professors Eva Nogales and James Hurley and began operations in February 2018. Our facility is used to collect image data of biological or soft material samples before it is processed using single particle or tomographic reconstruction methods Our staff offer consultation and training in specimen preparation, microscope operation, image acquisition, and data analysis. The facility’s instrumentation features automated data collection. Both single-particle micrographs and tomography tilt series can be collected.

Cal-Cryo@QB3-Berkeley instruments include: 

• Krios G2 300kV CryoTEM
  GATAN K3 Direct Electron Detector
  Volta Phase Plate, BIO Quantum Energy Filter
•  Krios G3i 300kV CryoTEM
  GATAN K3 Direct Electron Detector
  BIO Quantum Energy Filter
• Talos Arctica 200kV CryoTEM
  Gatan K3 Direct Electron Detector
• FEI Mark IV Vitrobot