QB3 High Throughput Screening FacilityBerkeley RIC

The High-Throughput Screening Facility (HTSF), located in the Li Ka Shing Center for Biomedical and Health Sciences, provides access to cell culturing space, automated liquid handling, automated plate reader and high-throughput, high-content microscopy instrumentation for screening experiments, along with tools for analysis and data visualization. Emphasis is on whole genome and sub-library siRNA screening from various sources but other high-throughput fluidics projects are welcome.

Instruments include:

• Combidrop/Multidrop, ThermoFisher
• V11 Bravo Automated Liquid Handler, Agilent with Inline Plate Washer and Sealer 
• BioMek NX with Span-8, Beckman-Coulter
• Muitiflo FX MultiMode Dispenser, Agilent/Biotek
• EVOS FL Color Imaging System, ThermoFisher
• Envision MultiMode Plate Reader, Perkin-Elmer
• Automated Confocal/Wide-field/Transmitted Light Microscope Opera Phenix, Perkin Elmer with Incubator and Robotic plate handler for Live Imaging
• UCB only: Lentiviral Packaging, Addgene CRISPR library amplification and verification Service
• UC only: Synthego CRISPR Knockout (KO) reagents