The Center for CRISPR Target Discovery (CCTD) provides comprehensive support for functional genomic experiments using CRISPR technology, including pooled library cloning and screening in mammalian cell cultures to identify gene targets for therapeutic development.
The College of Chemistry Glass Shop produces custom glassware for research. Services include design, consulting on glassware systems, and custom glassware fabrication, with experience in producing glass apparatus for physics, chemistry, materials engineering, and other research focuses.
Equipment includes glassblowing lathes, torches, kiln, diamond saw and other glass coldworking equipment.
The PEER Lab is located at the Richmond Field Station and has a long history of successfully providing the engineering community testing facilities, the staffing expertise to execute a given project, and the academic background to provide appropriate data analysis, design input and overall project management. The PEER-UC Berkeley Lab is available to write both academic style reports, along with AC156 and IEEE-693 reports submitted to regulatory agencies.
The College of Natural Resources' Inductively Coupled Plasma (ICP) Spectroscopy Facility has a Perkin Elmer 5300 DV optical emission ICP with auto sampler. The ICP can measure up to at least 21 different elements simultaneously. Users should estimate between 6 and 12 samples per hour plus a half hour warm up of the instrument. Applications include plant elemental analysis, soil extracts, silicon chip analysis, nanoparticles, rock fusions to name just a few.
The Earth and Planetary Sciences XRD, SEM, and Sample Preparation Labs provide users the opportunity to perform a wide range of analyses on samples including X-ray powder diffraction, SEM imaging, and EBSD textural analysis, EDS chemical analysis as well as all sample preparation required for analysis.
Equipment includes:
QB3 Genomics is a partnership between the Vincent J. Coates Genomics Sequencing Lab ( GSL – B206 Stanley Hall), the Functional Genomics Laboratory (FGL – 255 Weill Hall, formerly LSA), and the Computational Genomics Resources Facility (CGRL – 238 Koshland Hall). QB3 Genomics’ mission is to make genomics research tractable and affordable to UC Berkeley and the greater genomics community.
Provides all services related to the X-ray diffraction of single crystals and powders of small molecule compounds. Typically, X-ray diffraction is used to determine the solid-state molecular structure at the atomic level of crystalline samples, including absolute stereochemistry of chiral compounds. The facility currently houses state-of-the-art single crystal and powder X-ray diffractometers.
The Microanalytical Facility provides Elemental Analysis. Elemental analysis is a process where a sample of the some material (e.g. soil, water ,polymers, minerals, chemical compounds) is analyze for their chemical composition. There are a several analytical instruments in the facility:
The Cell Culture Facility, located in 336 Barker Hall, supports research by providing cell cultures (mammalian and insect), media, supplies and expertise on a recharge basis for more than 70 different laboratories on and off the main campus. The Facility provides cell cultures for researchers to take to their labs for experiments, provides media and materials for checkout, and also allows use of laminar flow hoods, incubators and microscope for researchers who are using cells provided by the Facility, to use on site. We also work with off-site companies.
The High-Throughput Screening Facility (HTSF), located in the Li Ka Shing Center for Biomedical and Health Sciences, provides access to cell culturing space, automated liquid handling, automated plate reader and high-throughput, high-content microscopy instrumentation for screening experiments, along with tools for analysis and data visualization. Emphasis is on whole genome and sub-library siRNA screening from various sources but other high-throughput fluidics projects are welcome.
Instruments include:
Serves all UC affiliated laboratories, academic institutions, government agencies and commercial businesses – providing Sanger sequencing, genotyping, high-throughput nucleic acid purification/quantification, PCR reaction cleanup, PCR products size selection, human cell line authentication, stem cell validation, and free (and paid) consultation services. Open access (for a fee) instruments include: Bio-Rad CFX96 Touch Real Time PCR System, Bio-Rad QX200 Droplet Digital PCR System, and an Agilent Technologies TapeStation 4200.
The Marvell NanoLab, located in the CITRIS headquarters building, Sutardja Dai Hall, includes more than 15,000 sq feet of Class100 and Class1000 cleanroom. The Marvell NanoLab is a shared research center providing more than 100 Principal Investigators and over 500 academic and industrial researchers a complete set of micro- and nano-fabrication tools. Please contact us if you are an academic or industrial researcher interested in the capabilities of the Marvell NanoLab.
The BNC is a fabrication and experimentation facility specializing in BioMEMS, microfluidic devices, electron/ion beam microscopy and nanofabrication. We are a core facility of the California Institute for Quantitative Biosciences and are open to university, national lab, and industry users.
Provides EM services to the Berkeley campus and the academic community at large. The EML houses both TEMs and SEMs and provides three types of service: (1) education and training, (2) equipment for trained users, and (3) full service microscopy for pilot studies. The EML specializes in cryopreservation via HPF/FS. All services are available to anyone with an interest in electron microscopy: graduate and undergraduate students, post-doctoral researchers, faculty, staff, and non-UC users.
A state-of-the-art light microscopy facility specializing in imaging live cell and live small model organisms (zebrafish, drosophila, c. elegans, etc.), laser-scanning and spinning disk confocal microscopy, multi-photon microscopy, fluorescence lifetime imaging (FLIM), super resolution (AiryScan and Fast Airyscan), light-sheet microscopy (SPIM), holographic patterned illumination for optogenetic studies, and slide scanning with histological stains and fluorescence.
The Cell and Tissue Analysis Facility (CTAF) provides the cell culture equipment and high-end instrumentation needed to grow and assess mammalian cells, tissues, proteins, and more from flow cytometry to multiplex ELISA to automated epifluorescence, confocal, and multiphoton imaging.
Instruments include:
The Flow Cytometry provides UC Berkeley and local researchers with access to cutting-edge High-Dimensional Fluorescence Activated Cell Sorting (Hi-D FACS) and analysis. The facility also provides flow cytometry instruments and expertise.
Promotes the understanding of the structures and dynamics of natural products, potential therapeutics, proteins, and nucleic acids at the atomic level. Instruments include a 900 MHz spectrometer equipped with a cryoprobe, along with several other NMR systems.
Provides routine access for liquid- and solid-state NMR experiments as well as diffusion and micro-imaging applications. PMRC Core is primarily used for determining critical aspects of molecular structure, for studying reaction mechanisms qualitatively and quantitatively, and materials characterization. Instruments are available at field strengths 300 MHz to 700 MHz with probes to study a broad-band of nuclei (1H-19F and 31P-103Rh). Further information can be found at: pmrc.berkeley.edu
Provides comprehensive proteomics services using mass spectrometry, determining the protein contents of samples as simple as gel bands or as complicated as whole cell extracts. The facility provides identification of post-translational modifications and relative quantitation as well as consultation regarding sample preparation and experimental design.
The facility provides proteomics services principally through mass spectrometry. Currently we have two Thermo LTQ XL linear ion trap mass spectrometers and a Thermo Fusion Lumos mass spectrometer.
Provides routine acquisition of mass spectra and accurate mass measurements of biological, organic, and inorganic compounds, as well as mass measurements of intact proteins, lipids, oligosaccharides, and non-covalent protein-protein and protein-ligand complexes and supramolecular coordination clusters. The facility also provides advanced proteomics analysis and operates an open access laboratory.