QB3 High Throughput Screening Facility (HTSF)Berkeley RIC

The High-Throughput Screening Facility (HTSF), located in the Li Ka Shing Center, provides access to cell culturing space, automated liquid handling, automated plate reader and high-throughput, high-content microscopy instrumentation for screening experiments, along with tools for analysis and data visualization. In addition to facilitating whole genome and sub-library siRNA/CRISPR screening for pathway discovery, it welcomes many other high-throughput fluidics projects such as daughter plating, multiwell plate mRNA or lentiviral dispensing, 96-well Seahorse prep, mammalian cell/organoid model validation studies, compound screening, multiwell organoid maintenance, staining, and imaging, etc. Contact Pingping He, pingpinghe@berkeley.edu for consultation!

Instruments include:

• Combidrop/Multidrop, ThermoFisher
• V11 Bravo Automated Liquid Handler, Agilent with Inline Plate Washer and Sealer
• BioMek NX with Span-8, Beckman-Coulter
• Muitiflo FX MultiMode Dispenser, Agilent/Biotek
• BenchSmart Electronic 96 channel Pipettor, Rainin, Benchtop
• MicroPro 300 Electronic 96 channel Pipettor, Rainin, In BSL2 Hood
• EVOS M5000 Color Imaging System, ThermoFisher
• Envision MultiMode Plate Reader, Perkin-Elmer
• Automated Confocal/Wide-field/Transmitted Light Microscope Opera Phenix, Perkin Elmer with Incubator and Robotic plate handler for Live Imaging
• UCB only: Lentiviral Packaging, Addgene CRISPR library amplification and verification service