Core Facility Type

The Flow Cytometry provides UC Berkeley and local researchers with access to cutting-edge High-Dimensional Fluorescence Activated Cell Sorting (Hi-D FACS) and analysis. The facility also provides flow cytometry instruments and expertise.

The Cell and Tissue Analysis Facility (CTAF) provides the cell culture equipment and high-end instrumentation needed to grow and assess mammalian cells, tissues, proteins, and more from flow cytometry to multiplex ELISA to automated epifluorescence, confocal, and multiphoton imaging.

Instruments include:

• Cell Culturing Facility for Mammalian Cell Culture with Biospherix Hypoxia Chambers
• Automated Epifluorescence/Live Imaging Microscopy and Analysis Workstation; ImageXpress Micro, Molecular Devices
• Lionheart FX/Live Imaging, Agilent/Biotek
• Multiphoton Photon Microscopy; Bruker/Prairie Technologies
• Confocal Microscopy, Bruker/Prairie Technologies
• SpectraMax M5 MultiMode Plate Reader, Molecular Devices
• Bio-Plex MAGPIX Multiplex Reader, BioRad
• Flow Cytometry; Attune NxT Acoustic Focusing Cytometer blue/red/violet/yellow, ThermoFisher
• CFX Connect qPCR, Thermal Cycler, and GenePulser Xcell Electroporator, BioRad
• Histology Suite with Cryostar NX50 cryostat, HM 430 Freezing Stage Sliding Microtome, and Paraffin Embedding (FFPE) and Sectioning, ThermoFisher 

This facility was formerly titled the QB3 Shared Stem Cell Facility.

The High-Throughput Screening Facility (HTSF), located in the Li Ka Shing Center for Biomedical and Health Sciences, provides access to cell culturing space, automated liquid handling, automated plate reader and high-throughput, high-content microscopy instrumentation for screening experiments, along with tools for analysis and data visualization. Emphasis is on whole genome and sub-library siRNA screening from various sources but other high-throughput fluidics projects are welcome.

Instruments include:

• Combidrop/Multidrop, ThermoFisher
• V11 Bravo Automated Liquid Handler, Agilent with Inline Plate Washer and Sealer 
• BioMek NX with Span-8, Beckman-Coulter
• Muitiflo FX MultiMode Dispenser, Agilent/Biotek
• EVOS FL Color Imaging System, ThermoFisher
• Envision MultiMode Plate Reader, Perkin-Elmer
• Automated Confocal/Wide-field/Transmitted Light Microscope Opera Phenix, Perkin Elmer with Incubator and Robotic plate handler for Live Imaging
• UCB only: Lentiviral Packaging, Addgene CRISPR library amplification and verification Service
• UC only: Synthego CRISPR Knockout (KO) reagents