Nicholas Ingolia

Research Expertise and Interest

Ribosome Profiling, translation, genomics

Research Description

Nicholas Ingolia is an Associate Professor in the Department of Molecular & Cell Biology.

Prof. Ingolia studies how cells dynamically control the translation, localization, and degradation of mRNAs, along with the cellular roles for this post-transcriptional control of gene expression. To do this, he develops innovative high-throughput experimental approaches that address key challenges in RNA biology. Early in his career, he developed ribosome profiling, a technique for comprehensive and high-resolution measurements of translation. More recently, he has focused on the regulatory RNA-binding proteins that drive post-transcriptional gene regulation. He developed APEX-seq, which reveals the composition and organization of RNA-protein complexes within living cells. To learn the functional consequences of these interactions, he has performed proteome-scale surveys that measure the regulatory effects of RNA-binding proteins and delineate the protein motifs responsible for their activity. His work has been recognized by awards including the RNA Society Mid-Career Award (2023). He has also extended his work to dissect the wider regulatory networks that impinge on translation in the cell. His CiBER-Seq approach offers a broadly applicable tool to analyze these networks through CRISPR-based screening and high-throughput sequencing.

In the News

Using CRISPR, new technique makes it easy to map genetic networks

CRISPR-Cas9 makes it easy to knock out or tweak a single gene to determine its effect on an organism or cell, or even another gene. But what if you could perform several thousand experiments at once, using CRISPR to tweak every gene in the genome individually and quickly see the impact of each?
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