The IGI Next Generation Sequencing Core (NGS Core) offers a growing range of services, from library construction to sequencing to complete experimental design and support. The sequencers that comprise the foundation of the NGS Core include two Illumina MiSeqs, an Illumina iSeq, and an Illumina NextSeq2000. Together, these instruments provide IGI members with a flexible array of options and enable the NGS Core to support a broad range of applications and projects simultaneously.
This facility has generated hundreds of transgenic mice and made numerous gene-targeted mice. Recently the facility has shifted to using Cas9/CRISPR-mediated gene targeting as this offers the ability to produce gene-targeted mice with unprecedented speed and ease.