Core Facility Type

Serves all UC affiliated laboratories, academic institutions, government agencies and commercial businesses – providing Sanger sequencing, genotyping, high-throughput nucleic acid purification/quantification, PCR reaction cleanup, PCR products size selection, human cell line authentication, stem cell validation, and free (and paid) consultation services. Open access (for a fee) instruments include: Bio-Rad CFX96 Touch Real Time PCR System, Bio-Rad QX200 Droplet Digital PCR System, and an Agilent Technologies TapeStation 4200. 

QB3 Genomics is a partnership between the Vincent J. Coates Genomics Sequencing Lab ( GSL – B206 Stanley Hall), the Functional Genomics Laboratory (FGL – 255 Weill Hall, formerly LSA), and the Computational Genomics Resources Facility (CGRL – 238 Koshland Hall). QB3 Genomics’ mission is to make genomics research tractable and affordable to UC Berkeley and the greater genomics community.

The VCGSL provides QC and sequencing services. We operate Illumina MiSeq, HiSeq, and NovaSeq platforms, as well as a PacBio Sequel II system and Oxford Nanopore Minion system. The GSL also provide PacBio and ONT library preps and high molecular weight extractions.

The FGL provides molecular biology services for next-generation sequencing projects, including library preparation, QC, purification, enrichment, and single cell (10X) services.

The CGRL provides shared computational infrastructure and technical support in collaboration with Berkeley Research Computing. The CGRL also provides consulting for bioinformatics and genomic analyses.

For more detailed information on services, submissions, and rates please visit the QB3 Genomics.

The High-Throughput Screening Facility (HTSF), located in the Li Ka Shing Center for Biomedical and Health Sciences, provides access to cell culturing space, automated liquid handling, automated plate reader and high-throughput, high-content microscopy instrumentation for screening experiments, along with tools for analysis and data visualization. Emphasis is on whole genome and sub-library siRNA screening from various sources but other high-throughput fluidics projects are welcome.

Instruments include:

• Combidrop/Multidrop, ThermoFisher
• V11 Bravo Automated Liquid Handler, Agilent with Inline Plate Washer and Sealer 
• BioMek NX with Span-8, Beckman-Coulter
• Muitiflo FX MultiMode Dispenser, Agilent/Biotek
• EVOS FL Color Imaging System, ThermoFisher
• Envision MultiMode Plate Reader, Perkin-Elmer
• Automated Confocal/Wide-field/Transmitted Light Microscope Opera Phenix, Perkin Elmer with Incubator and Robotic plate handler for Live Imaging
• UCB only: Lentiviral Packaging, Addgene CRISPR library amplification and verification Service
• UC only: Synthego CRISPR Knockout (KO) reagents